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標記核苷酸是生命(ming)科(ke)學研究的(de)關鍵要(yao)素，是分子生物學中檢測特定核苷酸序(xu)列的(de)有效工(gong)具，通(tong)過酶的(de)作用即可結合到DNA/RNA序列(lie)中，進行測序和(he)分析(xi)。由于(yu)出色(se)的安(an)全(quan)性(xing)、穩定性(xing)和(he)便捷性(xing)，生物素和(he)熒(ying)光(guang)標記是用于(yu)原位雜交、基因表達譜分析(xi)或電泳遷移(yi)率轉(zhuan)移(yi)分析(xi)(EMSA)的(de)(de)核(he)酸(suan)探針的(de)(de)最常見標(biao)記。

產品應用

核酸序列測定（Southern/Northern印記）

Hi-C技術，捕捉染色質構象

菌(jun)落與噬菌(jun)體，細胞與染色體的(de)原位雜交

基(ji)因表達分析

治病(bing)病(bing)原體檢測

遺傳病與腫瘤診斷

品(pin)名：Aminoallyl-dUTP-XX-ATTO-532 Rhodamine-12-dUTP 

貨號：NU-803-RHOX

Aminoallyl-dUTP-XX-ATTO532 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine.

Recommended Aminoallyl-dUTP-XX-ATTO532/dTTP ratio for PCR and Nick Translation: 30-50% Aminoallyl-dUTP-XX-ATTO532/ 50% dTTP

Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal produdct yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.

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